CRISPR Collection

Structures of immune cascade complexes from bacteria. These include CRISPR complexes from different bacteria,with guide RNA, target DNA, and the Cas1, Cas2, Cas3, and Cas9 nucleases. This collection of structures can be used to show how bacteria recognize and destroy viral DNA. This system has become an important tool for genome engineering.

iBiology has two nice videos on CRISPR-Cas9

All the structures below can be viewed in Molecule World on the iPad or Molecule World for iPhone.  

We have a tutorial here for exploring the CRISPR Cas9 system in Molecule World.

Bio-Link presented a webinar on Genome Engineering with CRISPR Cas9 on Sept 30th. 

Structures in the collection

Type Description Download structure
An MW Collection

Collections can be downloaded in one step and viewed in the iPad version of Molecule World.  Individual structures can be viewed in either version.

Download the entire collection of CRISPR structures by clicking the link in the next column. Download individual structures using the links below.

Binary Data CRISPR_2.mwc

Cas9 from Streptococcus pyogenes with CRISPR RNA about to cut to double-stranded target DNA.

Cas9 + CRISPR RNA + target DNA ready to cut

Binary Data mmdb_5F9R.cn3

Cas9 from Streptococcus pyogenes Cas9 bound to guide RNA and a single-stranded DNA target.

Cas9 + guide RNA + target DNA

Binary Data mmdb_4OO8.cn3

Cas9 from Streptococcus pyogenes with CRISPR RNA bound to double-stranded target DNA.

Cas9 + CRISPR RNA + target DNA

Binary Data mmdb_4UN3.cn3

A Cascade CRISPR surveillance complex from E. coli

Cascade complex + guide RNA

Binary Data mmdb_4TVX.cn3

Another structure of the Cascade CRISPR surveillance complex from E. coli

Cascade complex + guide RNA, another view

Binary Data mmdb_4U7U.cn3

E. coli CRISPR complex with guide RNA bound to target DNA.

CRISPR complex + RNA + target DNA

Binary Data mmdb_4QYZ.cn3

Cas1 and Cas2, nucleases from E. coli that chop up and store viral DNA.

Cas1 and Cas2

Binary Data mmdb_4P6I.cn3

Cas3, a nuclease from Thermobifida fusca that attacks viral DNA.

Cas3 nuclease

Binary Data mmdb_4QQW.cn3

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